A HPTLC Determination And Fingerprinting Of Bacoside A In Bacopa Monnieri And Its Formulation
A simple sensitive HPTLC method developed for the analysis of bacoside A in the plant Bacopa monnieri and in its commercial monoherbal capsule formulation. The stationary phase was precoated silica gel G60F254 (20x11cm, aluminum sheet). The mobile phase used was chioroform:methanol:water (18:9:0.6). The plate was scanned and quantified at 540nm for bacoside A. The method was validated in terms of linearity, accuracy and specificity. The proposed HPTLC method provides a faster and cost effective qualitative control for routine analysis of bacoside A in formulations containing Bacope monnieri saponins.