A Novel submicron emulsion system loaded with doxorubicin overcome multi-drug resistance in MCF-7/ADR cells
1The First Affiliated Hospital, Zhengzhou University, Zhengzhou-450 052, China 2Academy of Medical and Pharmaceutical Sciences, Zhengzhou University, Zhengzhou-450 052, China 3School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou-450 001, China
Y X Zhao School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou-450 001 China E-mail: [email protected]
The purpose of the present study was to develop the Solutol HS15-based doxorubicin submicron emulsion with good stability and overcoming multi-drug resistance. In this study, we prepared doxorubicin submicron emulsion, and examined the stability after autoclaving, the in vitro cytotoxic activity, the intracellular accumulation and apoptpsis of doxorubicin submicron emulsion in MCF-7/ADR cells. The physicochemical properties of doxorubicin submicron emulsion were not significantly affected after autoclaving. The doxorubicin submicron emulsion significantly increased the intracellular accumulation of doxorubicin submicron emulsion and enhanced cytotoxic activity and apoptotic effects of doxorubicin. These results may be correlated to doxorubicin submicron emulsion inhibitory effects on efflux pumps through the progressive release of intracellular free Solutol HS15 from doxorubicin submicron emulsion. Furthermore, these in vitro results suggest that the Solutol HS15-based submicron emulsion may be a potentially useful drug delivery system to circumvent multi-drug resistance of tumor cells.