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Abstract

A RP-HPLC method for simultaneous estimation of paracetamol and aceclofenac in tablets

Author(s): R Gopinath, S Rajan, SN Meyyanathan, N Krishnaveni, B Suresh
Department of Pharmaceutical Analysis, J. S. S. College of Pharmacy, Ootacamund-643 001, India

Correspondence Address:
S N Meyyanathan Department of Pharmaceutical Analysis, J. S. S. College of Pharmacy, Ootacamund-643 001 India E-mail: [email protected]


A simple, selective, rapid, precise and economical reverse phase HPLC method has been developed for the simultaneous estimation of paracetamol and aceclofenac from pharmaceutical dosage forms. The method was carried out on a Hichrom C18 (25 cm×4.6 mm i.d., 5 µ) column with a mobile phase consisting of acetonitrile:20 mM phosphate buffer (pH 5.0) (60:40 v/v) at a flow rate of 0.8 ml/min. Detection was carried out at 265 nm. Etoricoxib was used as an internal standard. The retention time of paracetamol, aceclofenac and etoricoxib was 4.75, 6.44 and 8.83 min, respectively. The developed method was validated in terms of accuracy, precision, linearity, limit of detection, limit of quantitation and solution stability. The proposed method can be used for the estimation of these drugs in combined dosage forms.



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