A validated high-performance liquid chromatograhy method for estimation of ferulic acid in asafoetida and polyherbal preparation
Department of Quality Assurance, Bharati Vidyapeeth's College of Pharmacy, Belapur, Navi Mumbai-400614, India
A P Jadhav Department of Quality Assurance, Bharati Vidyapeeth's College of Pharmacy, Belapur, Navi Mumbai-400614 India E‑mail: [email protected]
A high-performance liquid chromatography method was developed for the estimation of ferulic acid from asafoetida and a polyherbal preparation. The separation was carried out on HiQSil ODS C-18 column with a mobile phase of acetonitrile: 10% acetic acid (20:80 v/v). The developed method was validated as per International Conference of Harmonization guidelines for various parameters such as accuracy, precision, linearity, limit of detection, limit of quantification and specificity; and found to be reliable. Linear regression analysis showed a good corelation between peak area and concentration with a corelation coefficient r 2 =0.996 in the range 200-7000 ng/ml. The developed method can be utilized for standardization of herbal formulation comprising asafoetida.