An Improved HPLC method For Estimation Of Sennosides In Senna
A simple high performance liquid chromatographic (HPLC) method for estimation of sennosides A and B in senna has been established. The aqueous extract of senna was directly injected and separated using reverse phase Shim pack CLC-CN column with UV detector monitoring at 220 nm. Compounds were well resolved when mixture of 20 mM sodium citrate buffer (pH 4.5) and acetonitrile in the ratio of 9:1 was used as mobile phase at the flow rate of 1.5 ml/min. The minimum detectable limit was 0.05 Âµg. The chemical analysis method supported the result. The present method is more suitable than the earlier reported HPLC method for routine analysis of sennosides.