Antibacterial Activity of Marine Bacillus Substances Against V. cholerae and S. aureus and in vivo Evaluation Using Embryonic Zebrafish Test System
Biological Oceanography Division, Biotechnology lab, CSIR-National Institute of Oceanography, Dona Paula, Goa-403 004, India
Biological Oceanography Division, Biotechnology lab, CSIR-National Institute of Oceanography, Dona Paula, Goa-403 004, India Email: [email protected]
Cell free extracts of Bacillus subtilis subsp. spizizenii and B. thuringiensis isolated from hydrothermal vent region of Azorean Island Faial, North Atlantic Ocean showed antagonistic against V. cholera serogroup 01 and Staphylococcus aureus, respectively. The in vitro inhibitions of infectious pathogens by both B. subtilis subsp. spizizenii and B. thuringiensis was concentration dependent. The result of sensitivity to protease test showed considerable reduction of antibacterial activity for both Bacillus strains, indicating the proteinaceous nature of the antibacterial substances. Further, our results on antibacterial substance of heat resistant, proteinaceous nature and bacteriostatic mode of action was evaluated for the toxicity studies using zebrafish animal model, an alternative to the use of antibiotics test against infectious diseases. Bacillus antibacterial extracts showed no toxic effects with the Staphylococcus aureus–zebrafish embryo infection studies.