Bcl-2 expression alters the mitochondrial tri carboxyl acid pathway in hepatic ischemic and reperfusion induced necrosis and apoptosis in rat liver
1 Cellular Biology Laboratory, College of Pharmacy, IFTM, Lodhipur Rajput, Moradabad - 244 001, India 2 National Biotechnology Center, Indian Veterinary Research Institute, Izatnagar - 243 112, India 3 Birla Institute of Technology and Sciences, Pilani - 330 031, India
P Chattopadhyay Cellular Biology Laboratory, College of Pharmacy, IFTM, Lodhipur Rajput, Moradabad - 244 001 India E-mail: [email protected]
Ischemic and reperfusion injury leads to necrosis and apoptosis. Mitochondrial enzymes and antiapoptotic gene plays an important role in necrosis and apoptosis. The aim of this study was to investigate the role of Bcl-2 expression in alternations in mitochondrial energy regulation during hepatic ischemia and reperfusion and role in necrosis and apoptosis. Total 12 Wistar rats were divided into sham-operated control group (I) and ischemia and reperfusion group (II). Mitochondrial tri carboxylic acid cycles marker enzymes, respiratory marker enzymes, apoptotic cells, necrotic cells and Bcl-2 expression was measured. Number of necrotic and apoptotic cells were increased in ischemic and reperfusion group with reducing tri carboxylic acid cycles marker enzymes, respiratory marker enzymes and decreasing of Bcl-2 expression. On the basis of our findings it may be concluded that suppression of Bcl-2 gene, inhibition of tri carboxylic acid cycles and respiration rate, adenosine tri phosphate production in mitochondria is a pathophysiological consequences which provides a clue for necrosis and apoptosis in hepatic ischemic and reperfusion injury.