Bioactive Compounds from Morus indica as Inhibitors of Advanced Glycation End Products
DOS in Food Science and Nutrition, Manasagangotri, University of Mysore, Mysuru-570 006, India
DOS in Food Science and Nutrition, Manasagangotri, University of Mysore, Mysuru-570 006, India, E-mail: [email protected]
The present study aimed at identifying the active antiglycation constituents of G4 variety of Morus indica leaves. The bioactive compounds of G4 were identified and quantified using ultra-performance liquid chromatography/mass spectroscopy, isolated by preparative high performance liquid chromatography and characterized by Fourier-transform infrared spectroscopy, nuclear magnetic resonance and scanning electron microscopy. Based on results of the ultra-performance liquid chromatography/mass spectroscopy, extract with maximum bioactive compounds was chosen to study antiglycation property at different stages. The 80 % methanol extract of Morus indica leaves showed presence of 4 compounds, chlorogenic acid, ferulic acid, rutin and apigenin of which apigenin showed the maximum yield. This is the first report on the isolation of apigenin from the leaves of Morus indica G4 variety. Results obtained indicated that Morus indica leaf extract inhibited formation of advanced glycation end products. Also, scanning electron microscopy images indicated the protective effect against the formation of acanthocytes. These results provided evidence for the antiglycation effects of Morus indica leaves and suggested a potential role in management of diabetic complications.