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Abstract

Chloramphenicol - Induced In Vitro Bioproduction Of Hyoscyamine From Datura Stramonium Linn And Datura Innoxia Mill

Author(s): M. K Tailang, R. K Ray, M. D Kharya

The present work is aimed at enhancing hyoscyamine production in Datura stramonium and Datura innoxia through chloramphenicol supplementation of culture medium. One to 2 ppm chloramphenicol in medium enhanced the callus growth as well as hyoscyamine content up to 112% W/W compared to callus grown on control medium. Supplementation of medium with higher concentrations of chloramphenicol caused no further increase in hyoscyamine production. On the contrary callus growth as well as hyoscyamine content reduced markedly.