Comparative in vitro cytotoxic studies of novel 8-(4'/2'-Methoxy/Unsubstituted phenylcarbamoyl)bicyclo[3.3.1]nonane derivatives on ehrlich ascites carcinoma cell line
1Post Graduate and Research Department of Chemistry, Seethalakshmi Ramaswami College, India 2Department of Biochemistry, Srimad Andavan Arts and Science College, India 3Centre for Advanced Research in Indian System of Medicine (CARISM), Sastra University, India
Radha Natarajan Post Graduate and Research Department of Chemistry, Seethalakshmi Ramaswami College India E-mail: [email protected]
Novel bicyclo[3.3.1]nonane derivatives were synthesized by an efficient methodology from acetoacetanilide, 2-methoxy and 4-methoxyacetoacetanilides, 1,3,5-trinitrobenzene and triethylamine. The structures of the compounds were characterized by UV/Visible, FTIR, 1 H NMR and 2D-correlation spectroscopy analysis. The in vitro cytotoxic studies were performed using Ehrlich Ascites Carcinoma cell line by Trypan blue dye exclusion assay and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide cell proliferation assay. The IC 50 values of the 8-(4'-/2'-methoxy/unsubstituted phenylcarbamoyl)bicyclo[3.3.1]nonanes were found to be 110.65 µg/ml, 148.23 µg/ml and 151.71 µg/ml, respectively. Thus (4-methoxyphenylcarbomyl)bicyclo[3.3.1]nonane was more potent compared to other two bicyclic adducts.