All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Abstract

Concurrent estimation of clopidogrel bisulfate and aspirin in tablets by validated RP-HPLC method

Author(s): PK Shrivastava, PK Basniwal, Deepti Jain, SK Shrivastava
Department of Pharmaceutics, Banaras Hindu University, Varanasi-221 005, India

Correspondence Address:
P K Shrivastava Department of Pharmaceutics, Banaras Hindu University, Varanasi-221 005, India India E-mail: [email protected]


A simple, rapid, precise RP-HPLC method was developed for simultaneous estimation of aspirin and clopidogrel bisulphate in tablet dosage form used in the treatment of cardiovascular diseases. To achieve the maximum resolution, acetonitrile:50 mM potassium dihydrogen phosphate buffer:methanol, solution pH adjusted to 3, in the ratio 50:30:20; v/v was selected as mobile phase. This mixture was found to be appropriate allowing good separation of both the components at a flow rate of 1.5 ml/min and detection wavelength 240 nm. In these conditions clopidogrel bisulfate and aspirin were eluated at the 7.47 and 2.2 min. The linearity was found in the concentration range 1.5-7.5 and 3.5-15.0 µg/ml, respectively. All the analytical validation parameters were determined and found with in the limit as per ICH guideline, which indicates the validity of method.



Share this