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Abstract

Determination of Azithromycin in pharmaceutical dosage forms by Spectrophotometric method

Author(s): BN Suhagia, SA Shah, IS Rathod, HM Patel, KR Doshi
Department of Quality Assurance, L. M. College of Pharmacy, Navrangpura, Ahmedabad-380 009, India

Correspondence Address:
B N Suhagia Department of Quality Assurance, L. M. College of Pharmacy, Navrangpura, Ahmedabad-380 009 India E-mail: [email protected]


A simple and sensitive spectrophotometric method has been developed for determination of azithromycin in its pharmaceutical dosage forms. In the proposed method, azithromycin is oxidized with potassium permanganate to liberate formaldehyde, which is determined in situ using acetyl acetone, in the presence of ammonium acetate. A yellow coloured chromogen was obtained, having an absorption maxima at 412 nm. The method is found to be linear in the concentration range of 10-75 µg/ml, with regression coefficient of 0.9978. Various reaction parameters such as concentration of potassium permanganate and reagent, time required for oxidation, and maximum colour intensity were optimized. The method was validated, and can be used successfully to assay azithromycin in its pharmaceutical dosage forms viz. tablets, capsules, and injections.



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