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Abstract
Development and validation of HPTLC method for the estimation of almotriptan malate in tablet dosage form
Author(s): A Suneetha1, B Syamasundar2
1Department of Pharmaceutical Analysis, Hindu College of Pharmacy, Guntur-522 002, India 2Department of Chemistry/Pharmacy, Acharya Nagarjuna University, Guntur-522 510, India
Correspondence Address:
B Syamasundar Department of Chemistry/Pharmacy, Acharya Nagarjuna University, Guntur-522 510 India E-mail: profbsyamsundar@yahoo.co.in
1Department of Pharmaceutical Analysis, Hindu College of Pharmacy, Guntur-522 002, India 2Department of Chemistry/Pharmacy, Acharya Nagarjuna University, Guntur-522 510, India
Correspondence Address:
B Syamasundar Department of Chemistry/Pharmacy, Acharya Nagarjuna University, Guntur-522 510 India E-mail: profbsyamsundar@yahoo.co.in
A new, simple, precise and accurate high performance thin layer chromatographic method has been proposed for the determination of almotriptan malate in a tablet dosage form. The drug was separated on aluminum plates precoated with silica gel 60 GF 254 with butanol:acetic acid:water (3:1:1) was used as mobilephase. Quantitative analysis was performed by densitometric scanning at 300 nm. The method was validated for linearity, accuracy, precision and robustness. The calibration plot was linear over the range of 100-700 ng/band for almotriptan malate. The method was successfully applied to the analysis of drug in a pharmaceutical dosage form.
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