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Abstract
Development and validation of HPTLC method for the estimation of rizatriptan benzoate in bulk and tablets
Author(s): B Syama Sundar1, A Suneetha2
1Department of Pharmacy, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur - 522 510, India 2Department of Pharmaceutical Analysis, Hindu College of Pharmacy, Amaravathi Road, Guntur - 522 002, India
Correspondence Address:
B Syama Sundar Department of Pharmacy, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur - 522 510 India E-mail: profbsyamsundar@yahoo.co.in
1Department of Pharmacy, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur - 522 510, India 2Department of Pharmaceutical Analysis, Hindu College of Pharmacy, Amaravathi Road, Guntur - 522 002, India
Correspondence Address:
B Syama Sundar Department of Pharmacy, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur - 522 510 India E-mail: profbsyamsundar@yahoo.co.in
A new, simple high performance thin layer chromatographic method has been proposed for the determination of rizatriptan benzoate in a tablet dosage form. The drug was separated on aluminum plates precoated with silica gel 60 F 254 with dichloromethane-acetone-acetic acid 3:2:0.2(v/v/v) as mobilephase. Quantitative analysis was performed by densitometric scanning at 230 nm. The method was validated for linearity, accuracy, precision and robustness. The calibration plot was linear over the range 200-700 ng/band for rizatriptan benzoate. The method was successfully applied to the analysis of drug in bulk and marketed tablets.
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