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Abstract
Development Of A Sensitive HPLC Method For Determination Of Plasma Levels Of Nifedipine
Author(s): S. A Shah, S. S Savale, I. S Rathod, C. J Shishoo
As part of an ongoing programme on the development of sustained release formulation of nifedipine (20 mg tablet) and determination of bioequivalence, a specific, sensitive and simple HPLC method with UV detection for estimation of nanogram levels of nifedipine in plasma has been developed. Nitrendipine is used as an internal standard. With a simple one-step extraction of nifedipine from plasma, the method has a linearity range of 6 to 200 ng/ml and an average recovery of 77.48%. Nifedipine plasma concentration versus time profile is presented for the sustained release formulation under development (formulation T) and compared with the standard formulation (formulation S).
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