Enhancing Hepatoprotective Bioactives of Phyllanthus Amarus Through Immobilization by Growth Promoters and Media Changes
The present paper describes enhancement of lignans, phyllanthin and hypophyllanthin present in Phyllanthus amarus plant. Supplementation was done to enhance secondary metabolites by modifying the media and treatment with different growth promoters and abiotic elicitors to increase the content of hepatoprotective bioactives in immobilized cell cultures, after incubation for 21 days. MS medium was supplemented with gibberellic acid and to make whole process commercially viable, when coconut water, sugarcane juice and water-melon extract were treated, it was revealed that watermelon extract, enhances maximum phyllanthin and hypophyllanthin yield followed by sugarcane juice, coconut water and gibberellic acid after estimation by HPTLC. The present method was found to be accurate, economical and viable to enhance the content of phyllanthin and hypophyllanthin in P. amarus for large-scale commercial production.