Evaluation of immunomodulatory activity of the alkaloid fraction of Trichopus zeylanicus gaertn on experimental animals
1Department of Pharmaceutical Chemistry, J. K. K. Nattaraja College of Pharmacy, Namakkal District, Komarapalyam-638 183, India 2Department of Pharmaceutics, J. K. K. Nattaraja College of Pharmacy, Namakkal District, Komarapalyam-638 183, India
R S Bachhav Department of Pharmaceutical Chemistry, J. K. K. Nattaraja College of Pharmacy, Namakkal District, Komarapalyam-638 183 India E-mail: [email protected]
Trichopus zeylanicusGaertn, (Trichopodaceae) is also known as “Arogyappacha” meaning the greener of health by tribal inhabitants (Kani tribes). This plant used as health tonic and rejuvenator. The whole plant material of Trichopus zeylanicusis defatted and successively extracted with methanol. The alkaloid fraction of Trichopus zeylanicuswas obtained from methanol extract. Up to the dose of 2000 mg/kg b.w. per orally alkaloid fraction of Trichopus zeylanicusdid not show any mortality or toxicity. Immunomodulatory activity of alkaloid fraction of Trichopus zeylanicusGaertn was evaluated using various in vivomodels including neutrophil adhesion test, delayed type hypersensitivity reaction, and effect on hematological parameter like, total white blood cell's, red blood cell's and hemoglobin and cyclophosphamide induce immunosupression. Sheep red blood cells were used to immunized the animals. The percentage of neutrophils adhesion to the nylon fiber was dose dependently increased in alkaloid fraction of Trichopus zeylanicus75, 150 and 300 mg/kg, p.o treated groups (50.57, 52.99 and 54.21%), respectively compared to control group. A dose dependent potentiating of delayed type hypersensitivity reaction induced by sheep red blood cells was also observed from the alkaloid fraction of Trichopus zeylanicus. On chronic administration of alkaloid fraction of Trichopus zeylanicus (75, 150 and 300 mg/kg. p.o.) caused significant (P<0.001) increased in hematological parameter like, total white blood cell's, red blood cell's and hemoglobin. Alkaloid fraction of Trichopus zeylanicusalso prevented the myelosupression in mice treated cyclophosphamide (30 mg/kg, p.o.). The result of present investigation suggested that alkaloid fraction of Trichopus zeylanicus stimulate defense system by modulating several immunological parameters.