Extraction, Purification and Analysis of Sweet Compounds in Stevia rebaudiana Bertoni using Chromatographic Techniques
Department of Forest Products, Dr. Y. S. Parmar University of Horticulture and Forestry, Nauni, Solan-173 230, Department of Forestry, Mizoram University, Aizawl-796 004, India
Department of Forest Products, Dr. Y. S. Parmar University of Horticulture and Forestry, Nauni, Solan-173 230, India, E-mail: [email protected]
Stevioside and rebaudioside-A are two commercially important diterpenoid glycoside natural sweeteners present in Stevia rebaudiana Bertoni. Stevioside is the major component about 300 times sweeter than sugar, while rebaudioside-A is 1.6 to 1.8 times sweeter than stevioside. Some of the modern extraction and purification techniques developed have been found to be complex and expensive. Therefore, an attempt was made to extract and purify stevioside and rebaudioside-A using simple, easy and inexpensive thin-layer chromatography and column chromatography techniques. Dried leaves were extracted with methanol followed by purification through column chromatography and further analysis was done through high performance liquid chromatography. This extraction method provided a simple and cost effective route to obtain almost pure stevioside and rebaudioside-A.