All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Abstract

Extraction-Spectrophotometric Determination Of Certain-?-Lactam Antibiotics With Methylene Blue

Author(s): B. B Prasad, S Gupta

An extraction-spectrophotometric method was established for the determination of certain β-lactams, cefaclor, ampicillin and amoxycillin in pure and dosage forms using methylene blue (MB) as an analytical reagent. The method is based on the formation of a chloroform-extractable blue colored ion-association complex (MB cation-antibiotic anion) through the reaction of a β-lactam antibiotic with an excess of methylene blue at pH 9.5. Good agreement with Beer's law was found in the range of antibiotic concentration of 3.5-90.0 μg/ml with a detection limit of 3.0 μg/ml. The method is simple, precise and accurate for pure analyte with excellent recovery (97-102%); and also, this does not require any separation of soluble excipients in pharmaceutical preparations.