All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Abstract

HPLC estimation of berberine in Tinospora cordifolia and Tinospora sinensiss

Author(s): GV Srinivasan, KP Unnikrishnan, AB Rema Shree, Indira Balachandran
Phytochemistry Division, Centre for Medicinal Plants Research, Arya Vaidya Sala, Changuvetty, India

Correspondence Address:
G V Srinivasan, Phytochemistry Division, Centre for Medicinal Plants Research, Arya Vaidya Sala, Changuvetty, India


A high-performance liquid chromatographic method for the estimation of berberine in the stem of Tinospora cordifolia (Willd.) Miers. ex Hook.f. and Thoms. and Tinospora sinensis (Lour.) Merrill is described. The dried stems of T. cordifolia and T. sinensis were defatted with petroleum ether (60-80°). The marc was dried and further extracted with methanol. The concentration of berberine in methanol extract was determined using a C-18 reverse phase column with a mobile phase of acetonitrile:water (10:90 v/v) at a flow rate of 0.6 ml/min and with UV detection at 266 nm. TLC and HPLC comparison of both the species revealed significant variation in the chemical constitution of the two species. This observation becomes important in the context of the use of T. sinensis in place of the genuine drug T. cordifolia .



Share this