HPTLC standardization of Tinospora cordifolia using tinosporaside
Department of Pharmaceutical Sciences, Birla Institute of Technology, Mesra, Ranchi - 835 215, India
S Jha Department of Pharmaceutical Sciences, Birla Institute of Technology, Mesra, Ranchi - 835 215 India E-mail: [email protected]
A simple and reproducible high performance thin layer chromatography method for the determination of tinosporaside in Tinospora cordifolia was developed and is described. This method involves separation of compounds by TLC on pre-coated silica gel 60F 254 plates with a solvent system of toluene: acetone: water (5:15:1) and scanned using densitometric scanner in UV reflectance photomode at 220 nm. The linearity was observed in the range of 0.5 to 8 mg. The tinosporaside content of 0.40% w/w was observed in test sample. The average percentage recovery value of 99.24±0.49 was obtained. The proposed method being precise and sensitive can be used for detection, monitoring and quantification of tinosporaside in Tinospora cordifolia.