In vitro Inhibitory Effect of Lanostane Triterpenoids of Kadsura coccinea on the Human Immunodeficiency Virus Type-1 Protease
Key Laboratory of Enzyme and Protein Technology, VNU University of Science, 334 Nguyen Trai, ThanhXuan, 1National Institute of Medicinal Materials, 3B QuangTrung, Hanoi, 2School of Pharmacy, Haiphong University of Medicine and Pharmacy, 72A Nguyen Binh Khiem, Ngo Quyen, Haiphong, Vietnam
Key Laboratory of Enzyme and Protein Technology, VNU University of Science, 334 Nguyen Trai, ThanhXuan, Vietnam, E-mail: [email protected]
Human immunodeficiency virus type-1 is the causative pathogen of acquired immunodeficiency syndrome and its protease is one of the primary targets for human immunodeficiency virus/acquired immune deficiency syndrome therapy. In this study, two seco-lanostane triterpenoids, 3,4-seco-9βH-lanost-4(28),7,24-trien-3-oic acid and 24(E)-3,4-seco-9βH-lanost-4(28),7,24-trien-3,26-dioic acid isolated from the roots of Kadsura coccinea, were found to significantly inhibit human immunodeficiency virus-1 protease, with IC50 values of 1.0±0.03 µM and 0.05±0.009 µM, respectively. Neither compound was toxic to human embryonic kidney 293T cells at concentrations effective against human immunodeficiency virus-1 protease. Our findings indicate that these triterpenoids are potential candidates for development of antihuman immunodeficiency virus/acquired immune deficiency syndrome drugs.