Ion pair-HPLC method for the simultaneous estimation of quinapril and hydrochlorothiazide in tablets
Department of Pharmaceutical Analysis, College of Pharmacy, Coimbatore-641 044, India
M Gandhimathi Department of Pharmaceutical Analysis, College of Pharmacy, Coimbatore-641 044 India E-mail: [email protected]
A simple, precise and rapid HPLC method has been developed and validated for the estimation of quinapril and hydrochlorothiazide simultaneously in combined dosage form. The mobile phase used was a mixture of 0.1% v/v triethylamine (pH 3.5), containing 1 mM of hexane sulphonic acid: acetonitrile (30:70% v/v). The detection of quinapril and hydrochlorothiazide was carried out on photo diode array detector at 220 nm. Results of the analysis were validated statistically and by recovery studies. The proposed method can be successfully used to determine the drug contents of marketed formulation.