Phenolic Constituents with Antioxidant and Antiviral Activities from Phyllanthus urinaria Linnea
Guangdong Province Key Laboratory of Pharmacodynamic Constituents of Traditional Chinese Medicine and New Drugs Research, Institute of Traditional Chinese Medicine and Natural Product, College of Pharmacy, Jinan University, Guangzhou 510632, 1Xiamen Key Laboratory of Biotechnology of TCM, Xiamen Medical College, Xiamen 361000, 2National Key Special Department of Liver Diseases of TCM, Shenzhen Traditional Chinese Medicine Hospital, Guangzhou University of Chinese Medicine, Shenzhen 518033, China
Guangdong Province Key Laboratory of Pharmacodynamic Constituents of Traditional Chinese Medicine and New Drugs Research, Institute of Traditional Chinese Medicine and Natural Product, College of Pharmacy, Jinan University, Guangzhou 510632, China, E-mail: [email protected]
Phyllanthus urinaria Linnea is commonly used in folk medicine with considerable therapeutic utility. Sixteen phenols of Phyllanthus urinaria were isolated from ethanol extract and their structures were identified by spectroscopic data. Oxygen radical absorbance capacity, radical scavenging assay and HBsAg and HBeAg ELISA kits were used to evaluate antioxidant and antiviral activities. All the isolates showed significant antioxidant activity. Especially, isolates 4 and 5 had the oxygen radical absorbance capacity of 5.12 and 8.13 U/mol and strong radical scavenging activity with IC50 values of 5.50 and 5.55 μM, respectively. Additionally, these studies also demonstrated that the compounds 1, 2, 11, 12, and 16 inhibited secretion of HBsAg and HBeAg in HepG2.2.15 cells. This study suggested that the phenolic constituents of Phyllanthus urinaria possessed antioxidant activities and these might play an important role in antihepatitic effects.