Protective effect of gymnema sylvestre ethanol extract on high fat diet-induced obese diabetic wistar rats
1Department of Pharmacology, KIET School of Pharmacy, Ghaziabad-201 206, India 2Department of Pharmacology, Faculty of Pharmacy, Hamdard University, New Delhi-110 062, India 3Department of Pharmacology, Vardhman Mahavir Medical College, Safdarjung Hospital, New Delhi-110 029, India 4Department of Pathology, Vardhman Mahavir Medical College, Safdarjung Hospital, New Delhi-110 029, India
V Kumar Department of Pharmacology, KIET School of Pharmacy, Ghaziabad-201 206 India E-mail: [email protected]
Obesity is associated with numerous co-morbidities such as cardiovascular diseases, type 2 diabetes, hypertension and others. Therefore, the present study was planned to investigate the effect of water- soluble fraction of Gymnema sylvestre ethanol extract on biochemical and molecular alterations in obese diabetic rats. Diabetes was induced by single i.v. injection of streptozotocin (45 mg/kg) via tail vein. Obesity was induced by oral feeding of high fat diet for a period of 28 days in diabetic rats. Body weight gain, food intake, water intake, hemodynamic parameters (systolic, diastolic, mean arterial blood pressures and heart rate), serum biochemical parameters (leptin, insulin, lipid levels, apolipoprotein B and glucose), cardiomyocyte apoptosis (cardiac caspase-3, Na + /K + ATPase activity and DNA fragmentation) organs and visceral fat pad weight and oxidative stress parameters were measured. Oral treatment with water soluble fraction of Gymnema sylvestre ethanol extracts (120 mg/kg/p.o.) for a period of 21 days, resulted in significant reduction in heart rate, mean arterial pressure, serum leptin, insulin, apolipoprotein B, lipids, glucose, cardiac caspase-3 levels, Na + /K + ATPase activity and DNA laddering, visceral fat pad and organ's weight and improved the antioxidant enzymes levels in the high fat diet induced obesity in diabetic rats. The results of present study reveal that water soluble fraction of Gymnema sylvestre ethanol extract could be useful intervention in the treatment of obesity and type-2 diabetes mellitus.