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Abstract

Quantification of Zerumbone in Eleven Cultivars of Curcuma longa Using RP-HPLC

Author(s): P. R. Kshirsagar, N. V. Pawar1, S. P. Patil2, M. S. Nimbalkar2 and S. R. Pai3*
Department of Biotechnology, Shivaji University, Vidya Nagar, Kolhapur-416 004, Maharashtra State, 1Department of Botany, The New College, Kolhapur-416 004, 2Department of Botany, Shivaji University, Vidya Nagar, Kolhapur-416 004, 3Amity Institute of Biotechnology (AIB), Amity University, Mumbai-Pune Expressway, Bhatan, Post Somathne, Panvel, Mumbai-410 206, India

Correspondence Address:
Amity Institute of Biotechnology (AIB), Amity University, Mumbai-Pune Expressway, Bhatan, Post Somathne, Panvel, Mumbai-410 206, India, E-mail: [email protected]


Zerumbone has gained attention in cancer research due to its tumor suppressor potency. Present study aimed to investigate zerumbone content from eleven cultivars of Curcuma longa by reversed-phase high-pressure liquid chromatographic analysis. The fresh and dry samples were subjected for the reversed-phase high-pressure liquid chromatographic analysis of zerumbone including validation studies. Fresh samples revealed zerumbone content ranged from 0.383-2.179 mg/g while dry samples ranged from 3.209-8.333 mg/g. Dry samples reflected high zerumbone content compared to fresh samples. Among the different cultivars accessed Selam showed highest content of zerumbone on fresh weight basis while, Alleppey on dry weight basis. This study provided comprehensive information on zerumbone content in members of family Zingiberaceae and also quantified it in eleven Curcuma longa cultivars from India.



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