Role Of Amino Acids And Vitamins In The Production Of Alkaline Phosphatase And Erythromycin By Saccharopolyspora Erythraea
Resting cell experiments with washed mycelia of Saccharopolyspora erythraea were performed to study the effects of amino acids and vitamins on the biosynthesis of erythromycin and alkaline phosphatase at two pH optima. It was found that amino acids (glycine and L-leucine), and vitamins (pantothenic acid and ascorbic acid), each increased alkaline phosphatase as well as erythromycin formation by S. erythraea. A combination of the vitamins and the amino acids was found to give additive effect. The amino acids with the help of the vitamins exerted such additional effect on the enzyme and erythromycin biosynthesis.