Role of mitochondrial enzymes and sarcoplasmic ATPase in cardioprotection mediated by aqueous extract of Desmodium gangeticum (L) DC root on ischemic reperfusion injury
1School of Chemical and Biotechnology, SASTRA University, Thirumalaisamudram, Thanjavur - 613 402, India 2Department of Plant Biotechnology, Amala Cancer Research Center, Amalanagar, Trichur - 680 553, India
G A Kurian School of Chemical and Biotechnology, SASTRA University, Thirumalaisamudram, Thanjavur - 613 402 India E-mail: [email protected]
The present study investigate the protective effect of aqueous root extract of Desmodium gangeticum in preserving mitochondrial and sarcoplasmic ATPase during ischemia reperfusion injury. The isolated rat hearts in both drug and control group were subjected to warm ischemia (37°), followed by reperfusion with the Langendorff perfusion system. The aqueous root extract of Desmodium gangeticum (L) at a dose of 50 mg/kg body weight was found to be effective in the rat heart for the management of ischemic reperfusion injury. Physiological parameters were significantly (P<0.05) improved in drug treated rat hearts. Creatine phosphokinase in coronary perfusate found to be declined. Moreover, sarcoplasmic ATPase and mitochondrial enzymes were significantly (P<0.05) improved in drug treated rat hearts. In fact, histological analysis of the myocardium also suggested an improved ultra structure in Desmodium gangeticum treated rat heart. These results suggest that Desmodium gangeticum aqueous root extract can preserve the mitochondrial and sarcoplasmic ATPase in the myocardium, resulting in the improvement of cardiac function after ischemia reperfusion injury.