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Abstract

RP-HPLC analysis of aspirin and clopidogrel bisulphate in combination

Author(s): K Anandakumar, T Ayyappan, V Raghu Raman, T Vetrichelvan, A.S.K Sankar, D Nagavalli
Adhiparasakthi College of Pharmacy, Melmaruvathur - 603 319, India

Correspondence Address:
K Anandakumar Adhiparasakthi College of Pharmacy, Melmaruvathur - 603 319 India E-mail: [email protected]


A reverse phase high performance liquid chromatography method was developed for the simultaneous estimation of aspirin and clopidogrel bisulphate in formulation. The separation was achieved by octadecyl column (C 18 ) and acetonitrile:methanol:20 mM phosphate buffer at pH 3 (50:7:43 v/v) as eluent, at a flow rate of 1 ml/min. Detection was carried out at 240 nm. Quantitation was done by external standard calibration method. The retention time of aspirin and clopidogrel bisulphate was found to be 2.40 and 9.27 min, respectively. The method has been validated for linearity, accuracy and precision. Linearity for aspirin and clopidogrel bisulphate were in the range of 10-50 µg/ml for both the drugs. The mean recoveries obtained for aspirin and clopidogrel bisulphate were 100.86% and 100.20%, respectively. The developed method was found to be accurate, precise, selective and rapid for the simultaneous estimation of aspirin and clopidogrel bisulphate in capsules.



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