RPLC determination of tinidazole and diloxanide furoate in tablets
Department of Quality Assurance, Al-Ameen College of Pharmacy, Bangalore-560027, India
P.N.S Pai Department of Quality Assurance, Al-Ameen College of Pharmacy, Bangalore-560027 India E-mail: [email protected]
A simple reverse phase liquid chromatographic method has been developed and subsequently validated for simultaneous determination of tinidazole and diloxanide furoate. The separation was carried out using a mobile phase consisting of acetonitrile, methanol and 0.2 M potassium dihydrogen phosphate (pH 5) in the ratio 2:3:2.The column used was SS Wakosil-II C-18 with a flow rate of 1 ml/min and UV detection at 282 nm. The described method was linear over a concentration range of 10-70 mg/ml and 10-90 mg/ml for the assay of diloxanide furoate and tinidazole, respectively. The mean recovery was found to be 100-101% for tinidazole and 97-103% for diloxanide furoate when determined at three different levels.