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Abstract
Simultaneous RPHPLC determination of nimesulide and tizanidine in tablets
Author(s): Hemlata Nimje, SP Wate, DP Dharkar, Rachna Razdan
Nagpur College of Pharmacy, Wanadongri, Nagpur, India - 441 110, India
Correspondence Address:
S P Wate Nagpur College of Pharmacy, Wanadongri, Nagpur, India - 441 110 India
Nagpur College of Pharmacy, Wanadongri, Nagpur, India - 441 110, India
Correspondence Address:
S P Wate Nagpur College of Pharmacy, Wanadongri, Nagpur, India - 441 110 India
A simple, specific, accurate and precise reverse phase high pressure liquid chromatographic method has been developed for the simultaneous determination of nimesulide and tizanidine from tablets. The sample was analyzed using methanol: water in the ratio of 65:35, pH adjusted to 4.15 with orthophosphoric acid on an octadecylsilane C18 column. The effluent was monitored at 1.4 ml/min flow rate using 307 nm as detecting wavelength. The linear dynamic ranges for nimesulide and tizanidine were 0.2-1.0 mg/ml and 10-50 mg/ml, respectively. Coefficients of correlation obtained for nimesulide and tizanidine were 0.998 and 0.996 - respectively.
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