All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Abstract

Simultaneous RP-HPLC estimation of gatifloxacin and ornidazole in tablet dosage forms

Author(s): Sonali Paramane, Lata Kothapalli, Asha Thomas, AD Deshpande
Department of Pharmaceutical Chemistry, Dr. D. Y. Patil Institute of Pharmaceutical Sciences and Research, Pimpri, Pune - 411018, India

Correspondence Address:
Lata Kothapalli Department of Pharmaceutical Chemistry, Dr. D. Y. Patil Institute of Pharmaceutical Sciences and Research, Pimpri, Pune - 411018 India E-mail: [email protected]


The proposed method is a simple, accurate, precise, specific and rapid method for the simultaneous estimation of gatifloxacin and ornidazole in bulk and tablet formulations. A column in isocratic mode, with a mobile phase consisting of acetonitrile: 0.025 M potassium dihydrogen phosphate buffer (50:50 v/v) with 0.5% v/v of triethylamine and its pH adjusted to 3.0 with glacial acetic acid were used. The flow rate was set at 1.0 ml/min and UV detection was carried out at 300 nm. The retention time of gatifloxacin and ornidazole were 2.89±0.017 min and 4.21±0.022 min, respectively. Linearity of gatifloxacin and ornidazole were found in the range of 2-24 µg/ml and 5-60 µg/ml, respectively. The developed HPLC method was extended for dissolution studies. The dissolution testing was performed at 50 rpm and 100 rpm in 0.1 N hcL0 as dissolution medium by paddle method.



Share this