All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Abstract

Simultaneous UV spectrophotometric method for the estimation of cefuroxime axetil and probenecid from solid dosage forms

Author(s): SV Chaudhari, Ashwini Karnik, Anuradha Adhikary, RS Tandale, PR Vavia
Pharmaceutical Sciences Division, Mumbai University Institute of Chemical Technology, Nathalal Parikh Marg, Matunga, Mumbai-400 019, India

Correspondence Address:
P R Vavia Pharmaceutical Sciences Division, Mumbai University Institute of Chemical Technology, Nathalal Parikh Marg, Matunga, Mumbai-400 019 India E-mail: [email protected]


A simple, rapid and precise UV spectrophotometric method for simultaneous estimation of cefuroxime axetil and probenecid from combined solid dosage form has been developed. Cefuroxime axetil and probenecid interfere with the UV absorption of each other. However, as they show different absorption maxima, use of modified Beer's law for the simultaneous estimation of both the drugs was explored. As both the drugs have different pharmacopoeial in-vitro dissolution media, simultaneous equations were developed in both the systems. The accuracy and precision of the method was determined and validated statistically. The method showed good reproducibility and recovery with percent relative standard deviation less than 2%. This method was successfully applied for determining the assay and in vitro dissolution of a marketed formulation.



Share this