Spectrofluorimetric method for determination of citalopram in bulk and pharmaceutical dosage forms
Dept of Pharmaceutical Quality Assurance, Manipal College of Pharmaceutical Sciences, India
S G Vasantharaju Dept of Pharmaceutical Quality Assurance, Manipal College of Pharmaceutical Sciences, India India E-mail: [email protected]
A simple accurate, sensitive reproducible spectrofluorimetric method was developed for the analysis of citalopram in pure and pharmaceutical dosage form. Citalopram showed strong native fluorescence in 0.05 M sulphuric acid having excitation at 239 nm and emission at 300 nm. All parameters like the effect of different solvents, pH, dilutions, reaction time, temperature and effect of excipients were thoroughly investigated. The calibration graph was linear in the range from 0.100 to 0.900 mg/ml. The proposed method was statistically validated and successfully applied for analysis of tablet dosage forms. The percentage recovery was found to be between 99.08% to 99.28%.