Spectrophotometric determination of andrographolides in Andrographis paniculata nees and its formulation
1 Avance' Phytotherapies Pvt. Ltd. Sarkhej-Gandhinagar Highway, Ahmedabad - 382 210, India 2 K. B. Institute of Pharmaceutical Education and Research, Sector 23, Gandhinagar - 382 023, India 3 L. J. Institute of Pharmacy, Sarkhej-Gandhinagar Highway, Ahmedabad - 382 210, India
K Pundarikakshudu L. J. Institute of Pharmacy, Sarkhej-Gandhinagar Highway, Ahmedabad - 382 210 India E-mail: [email protected]
A simple, sensitive and reproducible spectrophotometric method was developed for the estimation of andrographolides in Andrographis paniculata ( Kalmegh ) and its formulation. The method is based on the condensation of g-unsaturated lactone ring of andrographolides and picric acid in alkaline medium which results in the formation of colored complex, that which could be measured at 494 nm. Linearity of the proposed method was found between 5-50 µg/ml. The powdered samples of A. paniculata procured from different sources were found to contain 0.9-1.5% w/w of andrographolides. Formulation containing A. paniculata showed 0.3% w/w of the andrographolides. Our studies clearly indicated that Baljet reagent can also be employed for estimation of andrographolides. The method was found to be reliable and precise and could be used for analyzing formulations containing andrographolides for regular quality control purposes.