Spectrophotometric determination of gatifloxacin in pharmaceutical formulations and biological samples
Department of Chemistry, Pharmaceutical Chemistry Division, Vellore Institute of Technology, Deemed University, Vellore-632 014, India
Lakshmi Sivasubramanian Department of Chemistry, Pharmaceutical Chemistry Division, Vellore Institute of Technology, Deemed University, Vellore-632 014 India E-mail: [email protected]
Three new simple and sensitive spectrophotometric methods in ultraviolet region have been developed for the determination of gatifloxacin in bulk drug, pharmaceutical preparations and biological samples. Gatifloxacin exhibited maximum absorbance at 289 nm (method A) with apparent molar absorptivity of 1.23×10 4 l/mol×cm when dissolved in sodium hydroxide; and maximum absorbance at 292 nm (method B) with apparent molar absorptivity of 1.71×10 4 l/mol×cm when dissolved in hydrochloric acid. Third developed method (method C) was based on the formation of yellow coloured chromogen with ferric chloride and potassium dichromate, which showed maximum absorbance at 352 nm with apparent molar absorptivity of 1.23 × 10 4 l/mol×cm. Beer's law was obeyed in the concentration range of 5-30 µg/ml. Results of analysis of all methods were validated statistically and by recovery studies.