Spectrophotometric estimation of total alkaloids from Cinchona officinalis stem bank and marketed formulations containing cinchona.
B.V. Patel Pharmaceutical Education and Research Development Centre, Thaltej, Ahmedabad-380054
M N Ravishankara B.V. Patel Pharmaceutical Education and Research Development Centre, Thaltej, Ahmedabad-380054
The alkaloids of stem bark of Cinchona officinalis and the marketed formulations containing the stem bark of Cinchona were estimated by spectrophotometric method using tropaeolin 'OO' for the formation of colour complex. In Cinchona stem bark, the alkaloids are bound to tannins. The method adopted for the extraction of alkaloids from the samples has the advantage of extraction of mainly the alkaloids and not the other interfering substances like tannins. The method of analysis was found to be sensitive, precise and accurate and it can be adopted for routine quality control purposes.