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Abstract
Spectrophotometric method for the determination of Cefoperazone sodium in pharmaceutical formulations
Author(s): M Senthilraja1, PN Sanjaypai2
1 Department of Pharmaceutical Chemistry, K. M. C. H. College of Pharmacy, Kalapatti Road, Coimbatore-641 035, India 2 Department of Quality Assurance, Al-Ameen College of Pharmacy, Hosur Main Road, Bangalore-560 027, India
Correspondence Address:
M Senthilraja Department of Pharmaceutical Chemistry, K. M. C. H. College of Pharmacy, Kalapatti Road, Coimbatore-641 035 India E-mail: rajdanish2k@rediffmail.com
1 Department of Pharmaceutical Chemistry, K. M. C. H. College of Pharmacy, Kalapatti Road, Coimbatore-641 035, India 2 Department of Quality Assurance, Al-Ameen College of Pharmacy, Hosur Main Road, Bangalore-560 027, India
Correspondence Address:
M Senthilraja Department of Pharmaceutical Chemistry, K. M. C. H. College of Pharmacy, Kalapatti Road, Coimbatore-641 035 India E-mail: rajdanish2k@rediffmail.com
A new simple and sensitive spectrophotometric method was developed on the basis of a colour reaction of cefoperazone sodium with Folin Ciocalteu's phenol reagent in presence of sodium carbonate, and it is stable for 20 min. The method is based on the formation of blue coloured chromophore that has an absorption maxima at 668 nm and obeys Beer's law in the concentration range of 8/40 µg/ml. Results of the analysis were validated statistically and by recovery studies. The method was found to be suitable for routine determination of cefoperazone sodium.
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