Studies of antiviral activity and cytotoxicity of Wrightia tinctoria and Morinda citrifolia
1 Arulmigu Kalasalingam College of Pharmacy, Anand Nagar, Krishnankoil, 626 190, India 2 Institute of Pharmacology, Madurai Medical College, Madurai-625 020, India 3 Molecular Medicine, Katholieke Universiteit-Leuven and IRC Kulak Kapucijnenvoer 33, Leuven B-3000, Flanders, Belgium 4 Raga Institute for Medical Research, Katholieke Universiteit-Leuven Minderbroederstraat 10, Leuven B-3000, Belgium
P Selvam Arulmigu Kalasalingam College of Pharmacy, Anand Nagar, Krishnankoil, 626 190 India [email protected]
Different extracts of leaf parts of Wrightia tinctoria and fruit powder of Morinda citrifolia have been studied against replication of HIV-1(IIIB) in MT-4 cells and HCV in Huh 5.2 cells. Chloroform extract of Wrightia tinctoria exhibited a maximum protection of 48% against the cytopathic effect of HIV-1(IIIB) in MT-4 cells. Fruit juice of Morinda citrifolia exhibited a displayed marked cytotoxic activity in lymphocyte (MT-4) cells (CC50: 0.19 mg/ml). The 50% effective concentration for inhibition of HCV subgenomic replicon replication in Huh 5-2 cells by Morinda citrifolia was 0.98 µg/ml and by chloroform extract of Wrightia tinctoria was 10 µg/ml. The concentration that reduced the growth of exponentially proliferating Huh 5-2 cells by 50% was greater than 50 µg/ml.