Study of the soil isolates for antimicrobial activity
Department of Pharmaceutical Biotechnology, J. S. S College of Pharmacy, Ooty-643 001, India
A R Srividya Department of Pharmaceutical Biotechnology, J. S. S College of Pharmacy, Ooty-643 001 India [email protected]
During the process of screening for a potent antimicrobial compound, a new strain was isolated from the soil sample of Thalaikunda village in Ooty, Tamil Nadu. That organism was name as NK 2 . It was found to be antagonistic to both bacterial and fungal test organisms. Production of antibiotic was more in a newly formulated broth. Antibiotic production reached maximum at the end of the 70 h of fermentation by stirred flask culture. The antimicrobial compound was extracted in n -butanol, ethyl acetate and methanol. Antimicrobial compound, which was produced by the soil isolate NK 2 did not showed cytotoxic activity on Vero cell lines.