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Abstract

Tissue culture studies on tribulus terrestris linn.

Author(s): R Zafar, J Haque
Deptt. of Pharmacognosy and Phytochemistry, Hamdard College of Pharmacy, Hamdard Nagar, New Delhi-110 062,

Correspondence Address:
R Zafar Deptt. of Pharmacognosy and Phytochemistry, Hamdard College of Pharmacy, Hamdard Nagar, New Delhi-110 062

The static culture of Tribulus terrestris Linn was established in Murashige and Skoog's1 medium supplemented with Indole acetic acid (IAA) 1 ppm, 2,4-dichlorophenoxy acetic acid (2, 4 D)1 ppm. and 25 ml of coconut water. The 8 months old callus was analysed for the presence of hecogenin and neotigogenin and were quantitatively estimated by spectrophotometric method.