Transfecting CHO-K1 Cells: Comparison of CaPO4, Electroporation and Lipoplex Method with In-house Prepared Polyplex
Department of Cell and Molecular Biology, B. V. Patel Pharmaceutical Education, Research and Development (PERD) Centre, Ahmedabad-380 054, B. R. D. School of Biosciences, Sardar Patel University, Vallabh Vidhyanagar-388 120, CPL Biologicals Pvt. Ltd., Ahmedabad-382 210, Sardar Patel University, Vallabh Vidhyanagar-388 120, India
3Sardar Patel University, Vallabh Vidhyanagar-388 120, India, E-mail: [email protected]
The extensive demand of large scale therapeutic protein production in Chinese Hamster Ovarian cell lines increased the need of developing efficient and cost effective transfection reagent. Polyethylenimine has recently emerged as an attractive alternative to currently employed conventional transfection methods. In the present report, we attempted to develop inexpensive and easy to prepare polyethylenimine-based transfection reagent and compared its transfection efficiency with calcium phosphate, electroporation and lipoplex mediated transfection methods. Our results indicated highest transfection efficiency with in-house prepared polyplexes as marked by maximum percentage of enhanced cyan fluorescence protein positive cells. These findings contribute towards the development of economical and effective solution for high protein expression in Chinese hamster ovarian system.