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Abstract
Validated high performance thin layer chromatography method for simultaneous estimation of ofloxacin and ornidazole in tablet dosage form
Author(s): M Gandhimathi, TK Ravi, Nilima Shukla
Department of Pharmaceutical Analysis, Sri Ramakrishna College of Pharmacy, 395, Sarojini Naidu Road, Coimbatore-641 044, India
Correspondence Address:
M Gandhimathi Department of Pharmaceutical Analysis, Sri Ramakrishna College of Pharmacy, 395, Sarojini Naidu Road, Coimbatore-641 044 India E-mail: gands72@yahoo.co.in
Department of Pharmaceutical Analysis, Sri Ramakrishna College of Pharmacy, 395, Sarojini Naidu Road, Coimbatore-641 044, India
Correspondence Address:
M Gandhimathi Department of Pharmaceutical Analysis, Sri Ramakrishna College of Pharmacy, 395, Sarojini Naidu Road, Coimbatore-641 044 India E-mail: gands72@yahoo.co.in
A simple, accurate and precise high performance thin layer chromatographic method has been developed for the estimation of ofloxacin and ornidazole simultaneously in tablet dosage form. The method employed silica gel 60GF254 precoated plates as stationary phase and a mixture of n-butanol: ethanol: ammonia (5:5:4 %v/v/v) as mobile phase. The plate was scanned and quantified at 295 nm using Camag TLC scanner. The method was validated for linearity, accuracy, precision, repeatability and specificity, proving its utility in estimation of ofloxacin and ornidazole in combined dosage form.
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