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Abstract
Validated High Performance Thin Layer Chromatography Method For Simultaneous Estimation Of Phenytoin Sodium, Phenobarbitone Sodium And Carbamazepine In Tablet Dosage Forms
Author(s): K. M Patil, S. L Bodhankar
A simple, specific, precise and rapid high performance thin layer chromatography method has been developed for estimation of phenytoin sodium, phenobarbitone sodium and carbamazepine simultaneous!y in tablet dosage form. In this method, standard solution and sample solution of phenytoin sodium, phenobarbitone sodium and carbamazepine were applied on precoated silica gel G60 F254 TLC plate and developed using a mixture of acetone:toluene (100:40 v/v) as mobile phase. The quantification was done by densitometry at 217 nm. This HPTLC system was quantitatively evaluated in terms of linearity, accuracy, precision, repeatability and specificity proving the utility in estimation of drug content in tablet dosage form.
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