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Abstract

Visible Spectrophotometric And HPLC Methods For The Estimation Of Sertraline Hydrochloride From Tablet Formulations

Author(s): I Singhvi, S.C Chaturvedi

One visible spectrophotometric and one HPLC method has been developed for the estimation of sertraline hydrochloride from tablet formulations. Developed visible spectrophotometric method is based on the formation of chloroform extractable coloured complex of drug with nitrosonaptho1. The coloured complex shows absorbance maxima at 441.5 nm. Beer's law was obeyed in the concentration of 20-100 μg/ml of scrtraline hydrochloride. Developed HPLC method was a reversed phase chromatoraphic method using Intertsil C18 column and methanol : acetate buffer (pH 2.8)::80:20 as mobile phase with detection at 220 nm. Caffeine was used as an internal standard and linearity was observed in the concentration range of 10-250 μg/ml of sertraline hydrochloride for HPLC method. Results of anlaysis for both the methods were validated statisically and by recovery studies.