Visible spectrophotometric methods for the estimation of metoclopramide hydrochloride in tablets
Shree S. K. Patel College of Pharmaceutical Education and Research, Ganpat Vidyanagar, Kherva-382 711, India
S A Patel Shree S. K. Patel College of Pharmaceutical Education and Research, Ganpat Vidyanagar, Kherva-382 711 India E-mail: [email protected]
Two simple, sensitive, accurate, and rapid visible spectrophotometric methods have been developed for the estimation of metoclopramide hydrochloride in tablets. Method A is based on the reaction of drug with 4-dimethyl amino-benzaldehyde (Ehrlich reagent) to yield a yellow colour Schiff's base, which shows maximum absorbance at 438 nm against reagent blank, while method B is based on diazotisation of primary amine group of metoclopramide hydrochloride with sodium nitrite and hydrochloric acid followed by coupling with β-napthol (in alkaline medium) to form a red colour dye, which shows maximum absorbance at 553 nm against reagent blank. Beer's law was obeyed in the concentration range of 10-100 µg/ml in method A and 1-10 µg/ml in method B. Results of the analysis were validated statistically and by recovery studies.