Wound Healing Related Biological Activities and Chemical Composition of Absolute from Hemistepta lyrata Bunge Flower
Department of Cosmetic Science, College of Life and Health Sciences, Hoseo University, Asan 31499, 1Departments of Physiology, School of Medicine, Konkuk University, Seoul 05029, Republic of Korea, 2College of Chemistry and Chemical Engineering, Hunan Institute of Science and Technology, Yueyang, Hunan 414006, China
Department of Cosmetic Science, College of Life and Health Sciences, Hoseo University, Asan 31499, China, E-mail: [email protected]
The Hemistepta lyrata Bunge flower has various biological and pharmacological effects, but effects of its absolute on skin regeneration or wound healing-linked responses remain unknown. In the present study, effects of Hemistepta lyrata Bunge flower absolute were explored on the proliferation and migration of normal human keratinocytes as well as the composition of absolute from Hemistepta lyrata Bunge flower was analysed using gas chromatography-mass spectrometry. Hemistepta lyrata Bunge flower absolute stimulated both proliferation and migration of the keratinocytes. It also enhanced the phosphorylation of extracellular signal-regulated kinase 1/2 and serine/threonine-specific protein kinase in human keratinocytes. Moreover, the Hemistepta lyrata Bunge flower absolute increased the sprout outgrowth of human keratinocytes. Gas chromatography-mass spectrometry analysis of Hemistepta lyrata Bunge flower absolute allowed identification of 16 compounds. These findings indicated that the absolute promoted the migration and proliferation of keratinocytes. Therefore, Hemistepta lyrata Bunge flower absolute might play a beneficial role in skin regeneration or wound healing by elevating migration and proliferation of keratinocytes.