Indian Journal of Pharmaceutical Sciences
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Scientific Publication of the Indian Pharmaceutical Association
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Year : 2002  |  Volume : 64  |  Issue : 1  |  Page : 76-78

Simultaneous Spectophotometric Estimation Of Isoniazid And Rifampicin From Combined Dosage Forms


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The present communication deals with the development of a simple, specific, sensitive, rapid and economical procedure for simultaneous estimation of rifampicin and isoniazid in a combined dosage form. The method is based on the native ultraviolet absobance maxima of the two chemotherapeutic agents. As both compounds do not interact chemically in phosphate buffered saline, two wavelengths 263 nm and 333 nm (λmax of isoniazid and λmax of rifampicin, respectively) were used. In addition, rifampicin also shows absorbance at 263 nm in phosphate buffered saline. Both the drugs obey Beer's law in the concentration range that was employed in the method.


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