Indian Journal of Pharmaceutical Sciences
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RESEARCH PAPER
Year : 2006  |  Volume : 68  |  Issue : 5  |  Page : 635-638

Simultaneous estimation of bisoprolol fumarate and hydrochlorothiazide in tablet dosage form by RP-HPLC method


1 Shri B. M. Shah College of Pharmacy, Modasa-383 315, India
2 L. M. College of Pharmacy, Ahmedabad-380 009, India
3 B. M. Shah College of Pharmaceutical Education and Research, Modasa-383 315, India

Correspondence Address:
L J Patel
Shri B. M. Shah College of Pharmacy, Modasa-383 315
India
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DOI: 10.4103/0250-474X.29633

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A simple, specific, accurate and precise reverse phase high performance liquid chromatographic method was developed for the simultaneous estimation of bisoprolol fumarate and hydrochlorothiazide in tablet dosage form. A lichrospher 100 C-18, 5 m column 20 cm 4.6 mm in isocratic mode, with mobile phase containing water, acetonitrile and tetrahydrofuran in proportion of 80:20:5 v/v/v were used. The flow rate was 1 ml/min, and effluent was monitored at 225 nm. The retention time of bisoprolol fumarate and hydrochlorothiazide were 1.48 0.02 and 4.72 0.03 min respectively, and the resolution factor was 9.0. Linearity values for bisoprolol fumarate and hydrochlorothiazide were in the range of 10-150 g/ml and 1-90 g/ml respectively. The limit of detection and limit of quantification for bisoprolol fumarate was found to be 3.5 and 8.5 g/ml respectively; and for hydrochlorothiazide, 0.4 and 0.9 g/ml respectively. The proposed method is accurate, precise, specific and rapid for simultaneous estimation of bisoprolol fumarate and hydrochlorothiazide in tablet dosage form.


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