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SHORT COMMUNICATION
Year : 2008  |  Volume : 70  |  Issue : 1  |  Page : 118-120
Synthesis and pharmacological evaluation of (6-substituted 4-Oxo-4 H -chromene-3 yl) methyl N-substituted aminoacetates


1 Medicinal Chemistry Research Lab, University College of Pharmaceutical Sciences, Kakatiya University, Warangal - 506 009, India
2 Vaagdevi College of Pharmacy, Ram Nagar, Kakatiya University, Warangal - 506 009, India

Date of Submission17-Jul-2006
Date of Decision21-Aug-2007
Date of Acceptance07-Feb-2008

Correspondence Address:
G Achaiah
Medicinal Chemistry Research Lab, University College of Pharmaceutical Sciences, Kakatiya University, Warangal - 506 009
India
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DOI: 10.4103/0250-474X.40348

PMID: 20390097

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   Abstract 

A series of the title compounds were synthesized and characterized by spectral data. All the compounds were evaluated for in vitro antihistaminic activity by inhibition of isotonic contractions induced by histamine on isolated guinea pig ileum and the compound 6-k showed significant activity. A few compounds have also been screened for in vivo bronchodilatory activity. These compounds exhibited significant protection against histamine-induced convulsions in guinea pig at the dose of 50 µmol.


Keywords: Chromone, bronchodilatory, antianaphylactic, asthma, antiallergic, antihistaminic


How to cite this article:
Gajbhiye A, Mallareddy V, Achaiah G. Synthesis and pharmacological evaluation of (6-substituted 4-Oxo-4 H -chromene-3 yl) methyl N-substituted aminoacetates. Indian J Pharm Sci 2008;70:118-20

How to cite this URL:
Gajbhiye A, Mallareddy V, Achaiah G. Synthesis and pharmacological evaluation of (6-substituted 4-Oxo-4 H -chromene-3 yl) methyl N-substituted aminoacetates. Indian J Pharm Sci [serial online] 2008 [cited 2014 Oct 30];70:118-20. Available from: http://www.ijpsonline.com/text.asp?2008/70/1/118/40348


Chromone moiety is a component of a number of biologically active substances of both synthetic and natural origin having medical significance [1] . Thus it is of great interest to medicinal chemist for molecular manipulation and pharmacological evaluation. Chromone is reported to have coronary spasmolytic [2] , bronchodilatory [3] , antiallergic [4] , antianaphylactic [4],[5] , platelet antiaggregatory [6] and anti-asthmatic [7] activities. 3-(Hydroxymethyl)-4 H -chromen-4-one is a key intermediate for the synthesis of many drugs [8],[9] . It can be prepared by the two reported methods. The first method involves reduction of 4-oxo-4H-chromen-3-carbaldehyde using sodium borohydride in the presence of aluminum chloride and borane in THF [10] , and the second method uses condensation of 1-(2-hydroxyphenyl)-2-(methylsulfinyl)ethanone/2′-hydroxy-2-(methylsulfinyl)acetophenone with formaldehyde, followed by thermal elimination of methylsulfinyl group [11] . The first method resulted in poor yields and formation of complex mixture which required tedious purification process. Hence we followed the second method. Disodium chromoglycate, which contains chromone nucleus, is well known for prophylaxis and treatment of asthma, and on the other hand several antihistamines posses basic nitrogen. Hence it was proposed to synthesize title compounds, taking care to preserve the chromone skeleton along with the substituent in the benzo group and also to introduce a basic N-substituted amino group at the acetate group, separating the chromone ring and basic nitrogen with four atoms. Such a molecular framework is expected to retain the mast cell stabilizing potency and H 1 -receptor blockade and can be useful as both prophylactic as well as therapeutic agent in allergic patients.

Melting points were determined in open capillary tubes and are uncorrected. IR spectra were recorded on Perkin-Elmer spectrum, Bx-I IR spectrometer, 1 H NMR on Jeol-300D (300 MHz) using TMS as internal standard and mass spectra on VG Micromass 7070H instrument. The title compounds were prepared from 3-hydroxymethyl-4-oxo-chromene [11] as depicted in Scheme 1.

As shown in Scheme 1, the key intermediates were prepared and identified based on the reported data [10],[11],[12],[13] . Compounds 2 were synthesized by refluxing appropriate 3-(hydroxymethyl)-4 H -chromen-4-one (1; 0.01 mol) with chloroacetyl chloride (0.01 mol) in dry benzene (20 ml) under anhydrous conditions, using calcium chloride guard tube, for 2 h. The product thus formed was filtered, washed with small portions of benzene to remove unreacted chloroacetyl chloride and dried. It was purified by recrystallization from petroleum ether and benzene mixture (1:1). Infrared spectrum of the compound (2a; KBr) showed characteristic absorption frequencies at: 1641 (C = O, pyran), 1768 (C = O, ester), 2950 (C-H, aliphatic), 3069 (C-H, aromatic) cm -1 , respectively. 1 H NMR spectrum of the compound (2a; CDCl 3 ) exhibited proton signals at (d, ppm): 4.10 (s, 2H, -CH 2 Cl), 5.16 (s, 2H,-CH 2 OCO), 7.27-8.13 (m, 4H, C-2,C-6,C-7,C-8), and 8.23-8.26 (dd, 1H, -C-5).

Compounds 3 were synthesized from appropriate (4-oxo-4 H -chromen-3-yl) methyl 2-chloroacetates (2; 0.01 mol) by refluxing them in dry acetone (30 ml) with appropriate amines (0.01 mol), freshly fused potassium carbonate (2.0 g) for 3 h. The reaction mixture was filtered off and residue was washed with little dry acetone. The solvent was removed under vacuum and the solid so obtained was purified by recrystallization using ethyl alcohol. Molecular formula of 3a C 17 H 14 N 2 O 4 (molecular weight 310.304) requires: C: 65.80; H: 4.55; and N:9.03%; found C: 66.02; H: 4.68; N: 9.03%. Infrared spectrum of the compound (KBr; 3a; R = H) showed absorption bands at: 3284 (NH), 1670 (C = O of ester), 1654 (C = O)) and 1605 (C = N of pyridine) cm -1 , respectively. 1 H NMR spectrum of the compound (3a; R = H), in CDCl 3 exhibited characteristic signals at (d, ppm):11.57(d, 1H, NH), 8.28 (d, 1H, C-5), 6.79-8.18 (m, 8H, aromatic) and 4.97 (s, 2H, -CH 2 O ), 4.98 (s, 2H, -CH 2 -N). Physical data of compounds 2a-2c and 3b-3o along with their invitro antihistaminic data are presented in [Table - 1]. The compounds were screened for in vivo bronchodilatory activity and were subjected for toxicity studies, and found to be nontoxic and safe in experimental animals (guinea pigs) up to a dose of 300 mg/kgbw (i.p.).

The test compounds were found to be free from CNS depression and did not cause any changes in the behavioural pattern of animals tested viz., impairment in awareness, mood and motor activity. Fifteen compounds of series 3a-o containing chromone ring attached to a basic moiety through four atom unit (-CH 2 -O-CO-CH 2 -) have been tested for their ability to inhibit histamine induced isotonic contractions in isolated guinea pig ileum ( in vitro model). Pheniramine maleate was used as standard (50 μg; 96.6% inhibition). The results are included in [Table - 1].

All of them exhibited significant antihistaminic activity at the concentration of 60 μg. Of all the compounds in the series, 3k (R = Cl; R1 = H, R 2 = 2-pyridyl) was found to be the most potent exhibiting 100% inhibition at the selected dose. Compound 3e with piperidine as basic moiety exhibited 49.3% inhibition and the activity increased to 94.8% with chlorine substitution at 6 position (3o; R = Cl, -NR 1 R 2 = piperidino). Also a methyl substituent at the same position (3j; R = CH 3, -NR 1 R 2 = piperidino) could cause 73.4% inhibition. However compound 3a with 2-pyridylamino group showed only 44.6% inhibition in the absence of a chlorine substituent (R = H). Thus both chlorine and methyl substituents being at 6 th position is contributing positively to enhance the activity. Compounds 3d and 3c with 4-alkyl piperazine as basic nucleus have been found to be more active than the compound with morpholino group (3b). As seen in case of piperidine containing compounds, chlorine substitution at 6 th position in compounds with peperazine also enhanced the activity.

Compounds 3k, 3n and 3o which showed greater in vitro potency, have been screened for in vivo bronchodilatory activity as well. All the three compounds exhibited significant protection against histamine induced convulsions in test animals (guinea pigs).

Among these three compounds, compound 3k (R = Cl; R 1 = H, R 2 = 2-pyridyl) showed relatively higher activity with 48.34% protection against 43.34% protection exhibited by standard drug (aminophylline), whereas 3o, 3n with piperidine and 4-ethylpiperazine groups showed only 38.34% and 35.99% protection respectively [Table - 2].

Selected compounds from the series (having greater in vitro potency) have been found to be devoid of toxicity and no mortality was observed upto a dose of 300 mg/kgbw (i.p.) in the experimental animals. Compounds 3k and 3o, which have comparable in vitro antihistaminic activity, differed in their in vivo bronchodilatory activity. Compound 3k with pyridyl moiety exhibited 48.34% protection against 38.34% protection shown by compound 3o (pyridine moiety) as compared to that of the standard aminophylline, which showed 43.34% protection.


   Acknowledgments Top


The authors express gratitude to the authorities of University College of Pharmaceutical Sciences for facilities and the authors AG and GA are thankful to AICTE, New Delhi for providing the financial assistance under QIP and for the research project, and Dr. H. S. Gour Vishwavidyalaya, Sagar (MP) for sponsoring AG.

 
   References Top

1.Ghosh CK, Ghosh C. Cycloaddition reactions of 1-benzopyran-4-ones. Indian J Chem 1997;36B:968-80.  Back to cited text no. 1    
2.Koo J. New classes of active central nervous system depressing and stimulating agents. J Org Chem 1961;26:635-6.  Back to cited text no. 2    
3.Wiley PF. Chromones and related compounds as bronchodilators. J Am Chem Soc 1952;74:4239-331.  Back to cited text no. 3    
4.Karale BK, Chavan VP, Hangarge RV, Mane AS, Gill CH, Shingare MS. Synthesis and characterization of some 3-styrylchromones. Indian J Heterocycl Chem 2001;10:233-4.  Back to cited text no. 4    
5.Nohara A, Umetani T, Sanno Y. Studies on antianaphylactic agents-I: A facile synthesis of 4-oxo-4H-1-benzopyran-3-carboxaldehyde by Vilsmeier reagents. Tetrahedron 1974;30:3553-61.  Back to cited text no. 5    
6.Singh G, Singh L, Ishar MP. 2-(N-methylanilino)-3-formylchromone: A versatile synthon for incorporation of chromone moity in a variety of heterocyclic systems and macrocycles through reactions with bifunctional nucleophiles. Tetrahedron 2002;58:7883-90.  Back to cited text no. 6    
7.Ellis GP, Shaw D. Benzopyrones. Part VIII. Mono- and Di-tetrazole-5-ylchromones.The Infrared Cyano-absorption of some 4-oxochromencarbonitriles. J Chem Soc Perkin Trans I 1972;6:779-83.  Back to cited text no. 7    
8.Bolos J, Gubert S, Anglada L, Planas JM, Burgarolas CJ, Castello JM, et al. 7-[3-(1-Piperidinyl)propoxy]chromenones as Potential Atypical Antipsychotics. J Med Chem 1996;39:2962-70.  Back to cited text no. 8    
9.Bolos J, Gubert S, Anglada L, Planas JM, Agut J, Princep M, et al. 7-[3-(1-Piperidinyl)propoxy]chromenones as Potential Atypical Antipsychotics. 2. Pharmacological Profile of 7-[3-[4-(6-Fluoro-1,2-benzisoxazol-3-yl)-piperidin-1-yl]propoxy]-3- (hydroxymethyl)chromen-4-one (Abaperidone, FI-8602). J Med Chem 1998;41:5402-9.  Back to cited text no. 9    
10.Nohara A, Umetani T, Ukawa K, Sanno Y. Studies on antianaphylactic agents. II. Oxidation and reduction of 4-Oxo-4H-1-benzopyran-3-carboxaldehydes. Synthesis of 4-Oxo-4H-1-benzopyran-3-carboxylic acids and 3-hydroxymethylchromones. Chem Pharm Bull 1974;22:2959-65.  Back to cited text no. 10    
11.Klutchko S, Cohen MP, Shavel J Jr, Von Strandtmann M. Heterocyclic synthesis with β - ketosulfoxides IV. Synthesis of 3-substituted chromones. J Heterocycl Chem 1974;11:183-8.  Back to cited text no. 11    
12.Becker HD, Mikol GJ, Russel G. Preparation and Pummerer Rearrangement of p-ketosulfoxidesl. IBID 1963;85:3410-4.  Back to cited text no. 12    
13.Bolos J, Loscertales T, Nieto J, Sacristan A, Ortiz JA. A new efficient synthesis of 3- hydroxymethyl)-4H-chromen-4-ones. J Heterocycl Chem 2000;37:1203-7.  Back to cited text no. 13    


    Figures

  [Figure - 1]
 
 
    Tables

  [Table - 1], [Table - 2]

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